Additive e ffects of hyperinsulinemia and ischemia on canine myocardial GLUT4 gene expression in vivo

VernacularTitle:胰岛素与低血流缺血刺激犬心肌GLUT4基因表达呈相加作用
Author: Ren-Fu YIN ¹ ; Jun ZHAO ; Jin-Ming CHEN ; Zong-Gui WU ; Shao-Hua QIU ; Yong-Mei WANG ; Rui-Mei WU
Author Information

1. Changzheng Hospital Second Milita ry Medical University
From: Academic Journal of Second Military Medical University 2001;22(2):115-117
CountryChina
Language:Chinese
Abstract: Objective: To investigate whether there is additi ve effects of hyperinsulinemia and ischemia on expression of canine myocardial G LUT4 gene in vivo. Methods: The expression of myocardial GLU T4 was determined by semiquantitative immunoblotting.The expression of GLUT4 mRN A was determined by semiquantitative Northern blotting. Results: Dramatic changes were seen in GLUT4 mRNA and GLUT4 expression in the ischemic hearts.After infusing insulin for 8 h,regional GLUT4 mRNA and GLUT4 levels in is chemic hearts were 2.5, 2.3-fold that of expression in normal hearts(P<0.01 ). Myocardial glucose uptake in ischemic hearts was increased by 4-fold when co mpared with normal hearts(P<0.01). Conclusion: There are not only additive effects of hyperinsulinemia and low-flow ischemia on canine myoc ardial GLUT4 mRNA and GLUT4 expression in vivo, but also increase of myocar dial glucose uptake. Enhanced GLUT4 expression may be an important protective m echanism by which myocardial cells enhance glucose uptake and metabolism during low-flow ischemia.