Effect of serum hyaluronic acid on the liver cryopreservation-reperfusion injury in rats
10.3321/j.issn:0258-879X.2001.01.023
- VernacularTitle:血清透明质酸在大鼠肝脏低温保存-灌注损伤中的作用
- Author:
Sui-Feng LÜ
1
;
Zhi-Ren FU
;
Guan-Nan XU
;
Meng-Long WANG
Author Information
1. Changzheng Hospital Second Milit ary Medical University
- From:
Academic Journal of Second Military Medical University
2001;22(1):77-79
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the relation between ser um hyaluronic acid (HA) concentration and cryopreservation-reperfusion injury. Methods: The animals were randomly assigned to 3 groups: (1 ) group A: the control; (2) group B: liver allografts were stored in lactated R inger's solution (0℃) for 2 h before implantation; (3) group C:liver allografts were stored in lactated Ringer's solution (0℃) for 4 h before implantation. Th e serum sample and liver specimen were taken up at 2 h and 4 h after transplanta tion to detect the concentration of HA, AST and LDH, and to get pathologic obser vation. Results: Serum HA increased earlier and decreased more s hortly than AST and LDH after transplantation in group A. Serum HA increased sig nificantly in group B and C, much higher than that in group A(P<0.01). The i njury of vascular endothelium and the disorder of hepatic sinuses and hepatic lo b ules were observed in group B and C. In the specimen of 4 h in group C, evident infiltration of inflammatory cell was present. Conclusion: Cryopreservation leads to injury of endothelial cell and reperfusion aggravat es this injury. The serum HA concentration indicates the degree of cold ischemia -reperfusion injury.
