Preparation of an adenovirus expressing cytosine deaminase and observat ion on its tumor-killing effect in vitro
10.3321/j.issn:0258-879X.2001.01.014
- VernacularTitle:含CD基因的腺病毒载体制备及体外抑瘤效果观察
- Author:
Guan-Rong DAI
1
;
Yuan-He WANG
;
Jun GAO
;
Long CUI
Author Information
1. 第二军医大学长征医院
- From:
Academic Journal of Second Military Medical University
2001;22(1):47-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare an adenovirus, Ad-CD, expressing bacterial cytosine deaminase(CD),and use it for tumor suicide gene therapy. Methods: We constructed a recomb ined adenoviral vector carrying CD or LacZ. 293 packaging cell was trans fected by the newly-constructed plasmid and generated the infectious virus. The n the adenovirus was used to infect C26 cell in vitro. Results: The growth of Ad-CD-infected cell was obviously surpressed after admini stration of 5-FC. Conclusion: CD gene carried by adenoviral vec tor is effective for tumor cell suicide gene therapy in vitro.
