Identification and biological characteristic of melatonin receptor in human embr yonic nervous system
10.3321/j.issn:0258-879X.2001.01.004
- VernacularTitle:人胚胎中枢神经系统褪黑素受体的鉴定及生物学特性
- Author:
Ying ZHAO
1
;
Fu-Yuan SHAO
;
Shu-Fen HE
;
Shu-Xun PENG
Author Information
1. Changzheng Hospital Second Military Medical Universi ty
- From:
Academic Journal of Second Military Medical University
2001;22(1):12-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To verify whether there exists melatoni n(Mel) receptor in human embryonic nervous system. Methods: Spec ific binding of Mel to embryonic brain and spinal cord was measured by radioliga nd binding assay. Results: 125 I-Mel binding s ites in optomeninx was the most, in eptochiasm and sniff ball was next; GTPγS d ose-de pendently inhibited the binding. Conclusion: The results demonst rate the presence of specific binding of Mel in human embryonic brain and spinal cord. GTPγS has some effect on 125 I-Mel specific binding,support ing the theory that Mel receptor is coupled to inhibitory G-proteins.
