Delineation of chromosome translocations by fluorescence in situ hybridization
10.3321/j.issn:0258-879X.2000.09.015
- VernacularTitle:用荧光原位杂交技术显示染色体易位
- Author:
Guan-Shan ZHU
1
;
Bartsch OLIVER
;
Mo-Bin WAN
;
Gillessen-Kaesbach GABRIELE
;
Passarge Erhard EB
Author Information
1. Shanghai Changhai Hospital
- Keywords:
chromosome structural aberration;
fluorescence in situ hybridization;
degenerate oligonucleotide-primed PCR
- From:
Academic Journal of Second Military Medical University
2000;21(9):853-856
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To delineate the G-banding-sug gested chromosome translocations by fluorescence in situ hybridization (FISH ) technique. Methods: Locus-specific probes, generated by degen erate oligonucleotide-primed PCR (DOP-PCR) technique from yeast artificial chr omosomes (YACs) mapping the regions in question, were used for FISH tests. Results: Among the 2 cases unresolved by G-banding, FISH confirm ed that one had a balanced translocation between chromosome 11 and chromosome 13 , the other had an unbalanced translocation between chromosome 6 and chromosome X.Conclusion: Because of its high sensitivity and specificity, FISH technique is a powerful adjunct to chromosome banding techniques, particula rly for the delineation of subtle chromosome rearrangement(s) and the origin of segment(s).