Delineation of chromosome translocations by fluorescence in situ hybridization

VernacularTitle:用荧光原位杂交技术显示染色体易位
Author: Guan-Shan ZHU ¹ ; Bartsch OLIVER ; Mo-Bin WAN ; Gillessen-Kaesbach GABRIELE ; Passarge Erhard EB
Author Information

1. Shanghai Changhai Hospital
Keywords: chromosome structural aberration; fluorescence in situ hybridization; degenerate oligonucleotide-primed PCR
From: Academic Journal of Second Military Medical University 2000;21(9):853-856
CountryChina
Language:Chinese
Abstract: Objective:To delineate the G-banding-sug gested chromosome translocations by fluorescence in situ hybridization (FISH ) technique. Methods: Locus-specific probes, generated by degen erate oligonucleotide-primed PCR (DOP-PCR) technique from yeast artificial chr omosomes (YACs) mapping the regions in question, were used for FISH tests. Results: Among the 2 cases unresolved by G-banding, FISH confirm ed that one had a balanced translocation between chromosome 11 and chromosome 13 , the other had an unbalanced translocation between chromosome 6 and chromosome X.Conclusion: Because of its high sensitivity and specificity, FISH technique is a powerful adjunct to chromosome banding techniques, particula rly for the delineation of subtle chromosome rearrangement(s) and the origin of segment(s).