Luminex-based Immunoassay for Organ Transplantation.
10.4285/jkstn.2015.29.2.54
- Author:
Hyeyoung LEE
1
;
Eun Jee OH
Author Information
1. Department of Laboratory Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea. ejoh@catholic.ac.kr
- Publication Type:Review
- Keywords:
Transplantation;
Luminex;
HLA antibody;
Multiplex bead immunoassay
- MeSH:
Antibody Specificity;
Immunoassay*;
Organ Transplantation*;
Sensitivity and Specificity;
Transplantation;
Transplants*
- From:The Journal of the Korean Society for Transplantation
2015;29(2):54-60
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Development of luminex-based solid phase assays enables advanced measurement of HLA antibody with sensitivity, specificity, and increasing knowledge of unacceptable antigens. In this review, we described the principle of the luminex-based assay and its current applications for organ transplantation including C1q assay, calculated panel reactive antibody, and virtual cross-matching. We also discussed the technical aspects and limitations for clinical utilization. The variables related to measurement of HLA antibody specificities and their clinical relevance remain unclear, therefore the interpretation of results requires comprehensive knowledge and clinical information in critical cases.
