Human Vascular Endothelial Growth Factor cDNA Cloning and Expression in Osteoblasts

Author: Cao YANG ¹ ; Shuhua YANG ; Shen QU
Author Information

1. Xiehe Hospital Tongji Medical College Huazhong University of Science and Technology
From: Journal of Huazhong University of Science and Technology (Medical Sciences) 2001;21(2):134-137
CountryChina
Language:Chinese
Abstract: Human vascular endothelial growth factor (VEGF) cDNA was amplified by nested polymerase chain reaction method from the HL60 cells. Then a pCD-hVEGF165 recombinant plasmid was constructed. Rabbit osteoblasts were transfected with pCD-hVEGF165 plasmid by lipofectin mediated gene transfer. The transient expressive results were detected by immunohistochemical method. It was observed that the expression of human VEGF gene was detected 72 h after transfecting distinctly.