The Clinic Study on Synchronous Detection of HCV RNA in the Plasma and PBMC of Hepatitis C Patients
- Author:
Jin YE
1
;
Wei LIU
;
Jinsong GUO
;
Linglan ZENG
Author Information
1. Xiehe Hospital Tongji Medical College of Huazhong University of Science and Technology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2001;21(2):110-111
- CountryChina
- Language:Chinese
-
Abstract:
In order to increase the positive detection rate of HCV RNA in the patients with chronic hepatitis C (CHC), RT-PCR was used to synchronously detect HCV RNA in the plasma and peripheral blood mononuclear cells (PBMC) of 583 CHC patients with a continuously elevated level of ALT for more than one year. The results showed that the positive detection rate of HCV RNA in the plasma of the CHC patients was 19.2 %, while 24.5 % in PBMC. It was demonstrated that the positive detection rate for HCV RNA in PBMC was obviously higher than that detected in plasma. To synchronously detect HCV RNA in PBMC by using RT-PCR can increase the positive detection rate of HCV RNA in the CHC patients.
