Effect of Diazepam on the Contents of Amino Acids and Free Radical during Ischemia/Reperfusion Injury
- Author:
Bo HU
1
;
Yuanwu MEI
;
Guirong WEI
;
Xiaoying QIU
;
Shenggang SUN
;
E'tang TONG
Author Information
1. Xiehe Hospital Tongji Medical College Huazhong University of Science and Technology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2001;21(2):102-104
- CountryChina
- Language:Chinese
-
Abstract:
The protective effect and mechanism of diazepam on ischemia neurons during cerebral ischemia and reperfusion were studied. Sixty-three Wistar rats were divided randomly into nine groups: control group (n=7), ischemia (is) groups including subgroups of is3h, is3-h/rep1-h, is3-h/rep2-h, is3-h/rep3-h(n=7 in each group), diazepam treated groups (10 mg/kg, i.p.), including subgroups of is3-h, is3-h/rep1-h, is3-h/rep2-h, is3-h/rep3-h (n=7 in each group) with Zea longa's animal model of middle cerebral artery occlusion. The comparison between the ischemia group and diazepam-treated group showed that diazepam could obviously decrease the production of glutamate, asparate, MDA and increase the synthesis and release of GABA, SOD and GSH-PX. It was concluded that diazepam exerted its protective effects on neurons through complex mechanisms of regulating the synthesis and release of excitotary/inhibitory amino acids and free radicals.
