The Assembly of a Novel Enzyme Biosensor for Aflatoxin B1 Detection
10.3969/j.issn.1671-8135.2008.03.008
- VernacularTitle:一种新的检测黄曲霉毒素B1的酶生物传感器的制作
- Author:
Da-Ling LIU
1
;
Yi SHEN
;
Jing ZHANG
;
Dong-Sheng YAO
Author Information
1. 暨南大学
- Keywords:
Aflatoxin B1;
Enzyme electrode;
Multi-walled carbon nanotubes
- From:
China Biotechnology
2008;28(3):44-52
- CountryChina
- Language:Chinese
-
Abstract:
A novel biosensor for aflatoxin B1 detecting has been reported. The biosensor electrode for AFB1 detecting was assembled by immobilized aflatoxin-oxidoreductase using open-ended multi-walled carbon nanotubes as matrix. Its linear range was between 0.16μM and 3.2μM. And if the specific anti-aflatoxin B1 antibody and aflatoxin oxidoreductase were both immobilized on the electrode with Multi-Walled carbon nanotubes, the detection limit of the modified electrode could be 16 nM with a 10 times improved sensitivity. The aflatoxin enzyme biosensor assembled this way strode one step forward its practical application.
