Cloning and expression of polycystin-1 intracellular region cDNA
- VernacularTitle:多囊蛋白-1胞内区cDNA的克隆与表达
- Author:
Rui-Ying ZHENG
1
;
Chang-Lin MEI
;
Ji-Fang MAO
Author Information
1. Changzheng Hospital Second Military Medical University
- From:
Academic Journal of Second Military Medical University
2001;22(4):313-315
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To obtain polycystin-1 intracellular region. Methods: cDNA of polycystin-1 intracellular region was generated by PCR and then cloned into pProEX Hta, which was prokaryotic expression vector. After verified by sequencing, the recombinant was transformed into E.coli host to express and purify the fusion protein by affinity chromatography. Results: 660 bp cDNA of polycystin-1 intracellular region and 2.6×104 fusion protein were obtained. Conclusion: The fusion protein containing polycystin-1 intracellular region is obtained and is helpful for preparing anti-polycystin-1 monoclonal antibody.
