Role of carboxypeptidase E in promoting the migration of lymphocytes through vascular endothelial cells
10.3760/cma.j.issn.0254-5101.2018.12.009
- VernacularTitle:羧肽酶E促进淋巴细胞跨血管内皮细胞迁移的研究
- Author:
Yaya PIAN
1
;
Jingjing NIE
;
Zhenxiang GAO
;
Chengshan XU
;
Yuxuan DU
;
Jihong HU
Author Information
1. 100730,北京医院 国家老年医学中心 卫生部临床检验中心
- Keywords:
Carboxypeptidase E;
Lymphocyte;
Vascular endothelial cell;
Transmigration
- From:
Chinese Journal of Microbiology and Immunology
2018;38(12):931-937
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism of carboxypeptidase E ( CPE ) in promoting the migration of lymphocytes and their subsets through vascular endothelial cells. Methods CRISPR/Cas9 technology was used to prepare cpe gene-knockout MS1 (Cpe-/-MS1) cells. Adhesion ability of lymphocytes to MS1 and Cpe-/-MS1 cells was analyzed with adhesion assay. Expression of adhesion molecules on these cells were detected by RT-PCR and flow cytometry. Transwell model was used to compare the difference in the transmigration of lymphocytes and their subsets through MS1 and Cpe-/-MS1 cells. Results Cpe-/-MS1 cells were successfully obtained. Under the stimulation of TNF-α, the adhesion ability of lymphocytes to MS1 cells was much better than that of Cpe-/-MS1 cells. Moreover, adhesion molecules expressed on MS1 cells were significantly more than those on Cpe-/-MS1 cells. The percentages of lymphocytes and their sub-sets that transmigrated through MS1 cells were significantly higher than those through Cpe-/-MS1 cells. Con-clusion CPE involved in the adhesion of lymphocytes to vascular endothelial cells and the transmigration of them through vascular endothelial cells, which was of great significance for understanding the migration of lymphocytes across vascular endothelial cells to peripheral lymph nodes.
