Cyclic guanosine monophosphate-adenosine monophosphate ( cGAMP) enhances the immune respon-ses against Helicobacter pylori in BALB/c mice
10.3760/cma.j.issn.0254-5101.2018.12.007
- VernacularTitle:cGAMP可显著增强BALB/c小鼠对幽门螺杆菌蛋白抗原的免疫应答
- Author:
Jing CHEN
1
;
Youxiu ZHONG
;
Chongfa TANG
;
Yu LIU
;
Xuewei WANG
;
Yanbin ZHANG
;
Ping WANG
;
Meiying LIU
;
Bo WEI
Author Information
1. 国药中生生物技术研究院有限公司第五研究室
- Keywords:
cGAMP;
Adjuvant;
Helicobacter pylori;
Vaccine
- From:
Chinese Journal of Microbiology and Immunology
2018;38(12):914-921
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the adjuvant activities of cyclic guanosine monophosphate-adenosine monophosphate ( cGAMP) in enhancing humoral and cellular responses against Helicobacter pylori ( H. pylori) . Methods BALB/c mice were immunized with the protein antigens including UreA, UreB and NapA of H. pylori in combination with cGAMP as the adjuvant on 0 d and 14 d by subcutaneous administra-tion. Then, the serum-specific antibody responses were evaluated by ELISA. Flow cytometry ( FCM) and enzyme-linked immunospot assay ( ELISpot) were used to detect the cellular immune responses occurred in spleen and mesenteric lymph nodes (MLN). Results Subcutaneous administration of protein antigens of H. pylori together with cGAMP induced strong humoral and cellular immune responses in BALB/c mice. The levels of serum-specific IgG antibodies induced by adding cGAMP as the adjuvant were significantly higher than those by immunizing with antigens alone. The levels of splenic IFN-γ-producing lymphocytes in re-sponse to H. pylori antigens and cGAMP immunization were significantly higher than those in the correspond-ing groups without using cGAMP. Conclusion By using cGAMP as an adjuvant, H. pylori antigens could elicit significantly stronger humoral and cellular immune responses in mice than those induced by the anti-gens only. As a stable small molecular compound with strong adjuvant activity, cGAMP has the potential to be used for the development of H. pylori vaccine.
