MiRNA-30a-3p inhibits proliferation and metastasis of hepatocellular carcinoma cells by regulating caspase 1-mediated pyroptosis
10.3760/cma.j.issn.1007-631X.2018.11.007
- VernacularTitle:miRNA-30a-3p通过调控Caspase1介导的焦亡抑制肝癌细胞增殖和转移
- Author:
Chenyang DU
1
;
Hu SONG
;
Xingxing WANG
;
Zhen WANG
;
Jianjun ZHANG
Author Information
1. 300192,天津医科大学一中心临床学院
- Keywords:
Carcinoma,hepatocellular;
MicroRNAs;
Neoplasm metastasis
- From:
Chinese Journal of General Surgery
2018;33(11):920-923
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of miRNA-30a-3p on inhibiting the proliferation,invasion and metastasis of HCC cells by targeting Caspase 1 involved in pyroptosis.Methods The qRT-PCR and immunohistochemical staining were used to detect the expressions of miRNA-30a-3p and Caspase 1 in HCC cells.SMMC-7721 cells were transfected with miR-30a-3p agonists,inhibitors and Caspase 1-specific inhibitors.Western blot was obtained to detect the expression of EMT-related proteins (N-cadherin,vimentin,snail,MMP-2) and Caspase 1,IL-18 and IL-1β.Plate clone assay,CCK-8 kit and Transwell were carried out to detect the proliferation and immigration of HCC cells.Results Caspase 1 was highly expressed (t =17.54,P ＜ 0.05) in HCC tissues.Overexpression of miR-30a-3p inhibited HCC cells proliferation and metastasis,while miR-30a-3p inhibition increased the proliferation and metastasis of HCC cells.Overexpression of miR-30a-3p decreased the expression of Caspase 1 (t =12.73,P ＜ 0.05) and inhibited the induction of pyroptosis,inhibiting the expression of IL-18 (t =7.32,P ＜ 0.05) and IL-1 β (t =7.32,P ＜0.05).When miRNA-30a-3p was inhibited,the cell viability of HCC was increased (F1 =9.57,P ＜0.05).When miRNA-30a-3p and Caspase 1 were inhibited together,the cell viability of HCC decreased (F2 =10.66,P ＜ 0.05).Conclusion MiRNA-30a-3p regulate cell pyroptosis through Caspase 1 pathway,inhibiting the proliferation,invasion and metastasis of HCC cells.
