Caspases Activation in Ultraviolet B-induced Apoptosis of G361 Human Melanoma Cell Line.
10.5021/ad.2000.12.4.243
- Author:
Kyoung Chan PARK
;
Jeong Gu LIM
;
Dong Seok KIM
;
Hyun Ok CHOI
;
Kyu Han KIM
;
Kwang Hyun CHO
;
Jai Il YOUN
- Publication Type:Original Article
- Keywords:
Melanoma cells;
Ultraviolet B;
Apoptosis;
Caspase
- MeSH:
Apoptosis*;
Blotting, Western;
Caspase 3;
Caspases*;
Cell Line*;
Flow Cytometry;
Humans*;
Melanocytes;
Melanoma*
- From:Annals of Dermatology
2000;12(4):243-246
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Ultraviolet B (UVB) irradiation can induce apoptosis of melanocytes and melanoma cells. However, mechanism of UVB-induced apoptosis of melanoma cells is not clarified yet. OBJECTIVE: Our purpose was to study the molecular mechanism of UVB-induced apoptosis of melanoma cells. METHODS: G361 lightly pigmented melanoma cells were analyzed for apoptotic mechanism by flow cytometry and western blotting. RESULTS: G361 melanoma cells showed apoptotic features with gradual increment of UVB doses by MTT and flow cytometry. Western blotting disclosed activation of caspase-3 and poly (ADP-ribose) polymerase (PARP) after UVB irradiation. CONCLUSION: In this study, we showed that UVB-induced apoptosis of melanoma cells is mediated by PARP activation which is induced by caspase cascade.
