Molecular Cloning of hSC2 Encoding 5α-reductase-like Protein.
10.5021/ad.2000.12.4.252
- Author:
Ho Chul SEO
;
Do Won KIM
;
Moon Kyu KIM
;
Jung Chul KIM
;
Sang Lip CHUNG
- Publication Type:Original Article
- Keywords:
5α-reductase;
Dermal papilla cDNA library;
Human synaptic glycoprotein SC2
- MeSH:
Adult;
Amino Acid Sequence;
Animals;
Blotting, Northern;
Cell Line;
Clone Cells;
Cloning, Molecular*;
DNA, Complementary;
Gene Library;
Humans;
Isoenzymes;
Liver;
Metabolism;
Molecular Weight;
Oligonucleotide Array Sequence Analysis;
Open Reading Frames;
Rats;
Testis;
Testosterone;
Tissue Distribution
- From:Annals of Dermatology
2000;12(4):252-258
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: The human homologue of the SC2 gene from a human dermal papilla cell cDNA library has been isolated and designated hSC2. HSC2 protein also shares similarity with 5 -reductase, a protein important in testosterone metabolism. OBJECTIVE: Prior to knowing the functions of hSC2 in dermal papilla, we cloned it and analyzed its relative expression levels in adult tissues and cancer cell lines. METHODS: hSC2 was isolated from low abundant clones in dermal papilla cDNA library using cDNA array hibridization method. Full-length clone was sequenced and we studied its expression in different tissues by Northern blot hybridization. RESULTS: Sequence data reveals a single open reading frame, encoding a putative hydrophobic protein with a calculated molecular weight of 36 kDa. Its deduced amino acid sequences are almost 97.4% identical to t4ose of rat protein. Northern blot hybridization shows that hSC2 cDNA recognizes a 1.35 kb transcript that was expressed in various epithelial and mesenchymal tissues including testis and liver. CONCLUSION: We have cloned and analysed tissue distributions of hSC2. It was interesting that it had homology with 5α-reductase isozymes. Further studies will be needed to understand the involvement of hSC2 in androgen hormone signaling.
