Endogenous protective mechanism of intestinal barrier injury induced by cardiopulmonary bypass in rats: the relationship with enteric glia cells
10.3760/cma.j.issn.0254-1416.2018.08.007
- VernacularTitle:体外循环诱发大鼠肠屏障损伤的内源性保护机制:与肠神经胶质细胞的关系
- Author:
Xiaoting YI
1
;
Chang CHANG
;
Yingjie SUN
Author Information
1. 110016,沈阳军区总医院麻醉科
- Keywords:
Cardiopulmonary bypass;
Intestinal mucosa;
Neuroglia
- From:
Chinese Journal of Anesthesiology
2018;38(8):921-924
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the relationship between endogenous protective mechanism of intestinal barrier injury induced by cardiopulmonary bypass ( CPB) and enteric glia cells in rats. Methods Forty-eight clean-grade adult male Sprague-Dawley rats, weighing 400-500 g, were divided into 2 groups (n=24 each) using a random number table method: sham operation group (group S) and CPB group. Rats were sacrificed at the beginning of CPB, 60 min of CPB, and 2 and 6 h after CPB, and the intestinal tissues were removed for examination of pathological changes ( by HE staining) and for determina-tion of the expression of ZO-1, occludin, glial fibrillary acidic protein ( GFAP ) and calcium-binding pro-tein ( S-100) and the positive expression of GFAP ( by immunohistochemical method) . Results Compared with group S, the expression of GFAP and S-100 was significantly up-regulated at 60 min of CPB and 2 and 6 h after CPB, the expression of ZO-1 and occludin was down-regulated (P<0. 05), the positive expres-sion of GFAP was enhanced, and the intestinal mucosal injury was marked in group CPB. Conclusion The enhanced activation of enteric glia cells may be involved in the endogenous protective mechanism of in-testinal barrier injury induced by CPB in rats.
