The effect of artesunate on the expression of HO-1 induced by ESAT-6/CFP-10 of mycobacterium tuberculosis
10.3760/cma.j.issn.1008-1372.2018.11.011
- VernacularTitle:青蒿琥酯对结核分枝杆菌ESAT-6和CFP-10诱导的HO-1表达影响
- Author:
Xiaohua MA
1
;
Yangen XIANG
;
Dongbin LIU
;
Jianhua PAN
;
Renhua FAN
;
Rong YU
;
Guomin SHI
Author Information
1. 410004,长沙市中心医院检验科
- Keywords:
Artemisinins/PD;
Bacterial proteins;
Antigens,bacterial;
Toll-like receptors;
Heme oxygenase-1
- From:
Journal of Chinese Physician
2018;20(11):1647-1650
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of artesunate (ASN) on the expression of Heme oxygenase-1 (HO-1) in THP-1 cells induced by the early secretory antigenic target-6 (ESAT-6) and culture filtrate protein-10 (CFP-10) antigens of Mycobacterium tuberculosis and to investigate its possible mechanism.Methods THP-1 ceils were cultured in vitro.The effects of ESAT-6 and CFP-10 on cell viability were detected by methyl thiazolyl tetrazolium (MTT) assay.THP-1 cells were pre-treated with or without ASN prior to incubation with or without ESAT-6 and CFP-10,the mRNA expression of HO-1 was detected by real time quantitative polymerase chain reaction (RT-qPCR) and Toll-like receptor 2 (TLR2) level was measured by Western blot.Results MTF assay showed that ESAT-6 and CFP-10 were non-toxic to cells in the range of 0-5 μg/ml.Compared with the control group,5 μg/ml ESAT-6 and 5 μg/ml CFP-10 could significantly increased the mRNA expression of HO-1 (P ＜ 0.05).In addition,20 μg/ml ASN could significantly enhance the mRNA expression of HO-1 induced by ESAT-6 and CFP-10,and inhibit the expression of TLR2 induced by ESAT-6.Conclusions ASN in combination with ESAT-6 or CFP-10,may have potential value in treatment of pathogen-associated inflammatory diseases.
