LncRNA RP11-316M1.12 promotes invasion of breast cancer cells
10.3760/cma.j.issn.1008-1372.2018.11.003
- VernacularTitle:LncRNA RP11-316M1.12促进乳腺癌细胞侵袭转移
- Author:
Lejuan SHI
1
;
Xiaoting JIA
;
Liyun LUO
;
Bolin LIU
;
Guopei ZHENG
Author Information
1. 510095 广州医科大学附属肿瘤医院,广州医科大学肿瘤研究所,广州恶性肿瘤治疗转化医学重点实验室
- Keywords:
RNA,untranslated;
Breast neoplasms;
Cell migration assays;
In vitro
- From:
Journal of Chinese Physician
2018;20(11):1608-1612
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role and mechanism of long non-coding RNA (lncRNA)RP11-316M1.12 in breast cancer cells.Methods Bioinformatics analysis was performed to varify RP11-316M1.12 expression pattern in breast cancer tissues and normal tissues.Quantitative real time polymerase chain reaction (qRT-PCR) assay was used to check the expression level of RP11-316M1.12 in breast cancer cells and tissues.Further,the correlationship between RP11-316M1.12 expression and clinical paremeters of breast cancer was analysed according to RP11-316M1.12 level.RP11-316M1.12 was overexpressed in MCF-7 cells,and RP11-316M1.12 was knocked down in MDA-MB-231 cells.Transwell method was used to detect the invasive ability of these cells.Western blot was used to detect the expression of epithelialmesenchymal transition (EMT) markers in these cells.Results Gene Expression Profiling Interactive Analysis (GEPIA) database suggested that RP11-316M1.12 was highly expressed in breast cancer tissues than that in normal tissues.The similar results were got in 65 cases of breast cancer tissues and 23 cases of normal tissues by qRT-PCR assay.Meanwhile,we found that RP11-316M1.12 was enhanced in breast cancer cells than that in normal epithelial cell and RP11-316M1.12 expression is related to TNM stage and distant metastasis in breast cancer.Transwell assay demonstrated that RP11-316M1.12 significantly enhanced breast cancer cells invasion.Mechanismly,over expression of RP11-316M1.12 can remakably downregulated E-cadherin,enhanced ZEB1 and Vimentin expression in these cells.Conclusions RP11-316M1.12 was enhanced in breast cancer,and RP1 1-316M1.12 could accelerate invasion of breast cancer cells.
