Effect of growth factors on the expression of proto-oncogenes c-fos and c-myc in FRTL-5 cell line.
10.3346/jkms.1995.10.3.155
- Author:
Hwan Young YOON
1
;
Seung Keun OH
;
Ka Hee YI
;
Bo Youn CHO
;
Chang Soon KOH
Author Information
1. Department of Surgery, Seoul National University College of Medicine, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Growth factors;
c-fos;
c-myc;
signal transduction system
- MeSH:
Animal;
Cell Division/drug effects/genetics;
Cell Line/cytology/physiology;
Gene Expression/drug effects/immunology;
Graves' Disease/immunology;
Growth Substances/genetics/*pharmacology;
Immunoglobulin G/pharmacology;
Insulin-Like Growth Factor I/pharmacology;
Myxedema/immunology;
Proto-Oncogene Proteins c-fos/*genetics;
Proto-Oncogene Proteins c-myc/*genetics;
RNA/analysis;
Rats;
Rats, Inbred F344;
Support, Non-U.S. Gov't;
Thyroid Gland/cytology;
Thyrotropin/pharmacology;
Time Factors
- From:Journal of Korean Medical Science
1995;10(3):155-163
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was performed to prove the hypothesis that oncogene expressions would have the same patterns with those of cellular growth to growth factors in FRTL-5 cells. Ribonucleic acids of FRTL-5 were extracted at 15', 30', 60' and 120' after administration of growth factors to quiescent FRTL-5, and blotted to the nitrocellulose membrane. They were hybridized with radiolabelled c-fos, c-myc and beta-actin probes. Hybridized dot blots were autoradiographed and the amount of radioactivity was measured by densitometry. Densitometric readings were used as the indices of oncogene expressions. Expressions of c-fos and c-myc were more prominent in combined administrations of TSH (10 mU/ml) and IGF-I (100 ng/ml) or IgG of Graves' disease (Graves' IgG; 1 mg/ml) and IGF-I than in combined administration of TSH and Graves' IgG. IgG of primary myxedema suppressed oncogene expressions by TSH or Graves' IgG, but not by IGF-I. From the above results, it was suggested that expressions of c-fos and c-myc to growth factors would have similar patterns with those of cell growth to growth factors in FRTL-5, and the actions of TSH and Graves' IgG would be manifested through same signal transduction system, but IGF-I would be manifested by its own.
