Identification of CD44 splice variant in Korean colorectal cancers and cell lines.
10.3346/jkms.1995.10.3.169
- Author:
Seong Il SUH
1
;
Won Ki BAEK
;
Jong Wook PARK
;
Ok Suk BAE
;
Min Ho SUH
;
Byung Kil CHOE
Author Information
1. Department of Microbiology, School of Medicine, Keimyung University, Taegu, Korea.
- Publication Type:Original Article
- Keywords:
CD44 splice variant;
Colorectal cancer
- MeSH:
Adenocarcinoma/*genetics;
Adult;
Aged;
Antigens, CD44/*genetics;
Base Sequence;
Blotting, Southern;
Colorectal Neoplasms/diagnosis/*genetics;
DNA Primers;
Electrophoresis, Agar Gel;
Feces/chemistry/cytology;
Female;
Gene Expression Regulation, Neoplastic/genetics;
Human;
Male;
Middle Age;
Molecular Sequence Data;
Polymerase Chain Reaction;
RNA Splicing/*physiology;
RNA, Messenger/analysis;
Tumor Cells, Cultured/*physiology;
Tumor Markers, Biological
- From:Journal of Korean Medical Science
1995;10(3):169-175
- CountryRepublic of Korea
- Language:English
-
Abstract:
CD44 is a glycoprotein expressed in a wide variety of cell types. Recently expression of some alternatively-spliced variants of CD44 transcripts (CD44v) has been suggested to play a potential role in tumor metastasis and the detection of CD44v containing exon 6 to 11 may be helpful for the diagnosis of cancers. Expressions of CD44v containing exon 6 to 11 were investigated in 20 human colorectal cancer samples, peripheral blood leukocytes isolated from colorectal cancer patients, and 4 colorectal cancer cell lines using reverse transcription-polymerase chain reaction and Southern blot analysis. The standard form of CD44 transcripts was expressed in all samples tested. CD44v containing exon 6 to 11 was expressed in 18 cases of colorectal cancers (sensitivity = 90%), 3 out of 4 cell lines, and one normal tissue (specificity = 95%). These results suggest that the expression of CD44v containing exon 6 to 11 can be regarded as tumor specific and that this marker may be helpful for the early diagnosis of colon cancers, if specimens from the early stage are available.
