Characteristics of etiology and drug and disinfectant resistant genes of Yersinia pestis in natural plague foci of Haixi Prefecture, Qinghai Province
10.3760/cma.j.issn.2095-4255.2019.01.005
- VernacularTitle:青海省海西州鼠疫自然疫源地鼠疫菌病原学及耐药、耐消毒剂基因特征分析
- Author:
Sheng LI
1
;
Xiaoyan YANG
;
Jian HE
;
Cunxiang LI
;
Haoming XIONG
;
Youquan XIN
;
Juan JIN
;
Qi ZHANG
;
Jixiang BAI
;
Baiqing WEI
;
Ruixia DAI
Author Information
1. 青海省地方病预防控制所鼠疫菌专业实验室
- Keywords:
Yersinia pestis;
Etiology;
Drug and disinfectant resistance genes
- From:
Chinese Journal of Endemiology
2019;38(1):15-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the biological characteristics of Yersinia pestis strains in Haixi Prefecture,Qinghai Province,in order to provide a scientific basis for plague prevention and control in future.Methods Totally 181 strains were separated from variety kinds of host in Haixi Prefecture,Qinghai Province from 1957 to 2011,and these strains were conducted biochemical test,virulence factor evaluation,plasmid analysis,different region (DFR) genotyping,drug and disinfectant resistant genes detection;79 of the 181 strains were examined by toxicity test and classified according to the criteria (minimum lethal dose:MLD≤ 10 000 was velogenic strain,10 000 ＜ MLD ＜ 100 000 was moderate virulence strain,MLD ≥ 100 000 was hypovirulent strain).Results According to six biochemical typing about gelatin candy,rhamnose,maltose,melibiose,glycerin and denitrification,the 181 strains of Yersinia pestis were antique biovar and Qing-Tibet Plateau ecotype.Aproportion of 81.22％ (147/181) of Yersiniapestis strains contained all the four virulence factors (F1,Pst Ⅰ,VW,Pgm).Totally 63.54％ (115/181) of the strains contained 3 kinds of plasmid-6 × 106,45 × 106,and 52 × 106;31.49％ (57/181) of the strains contained 3 kinds of plasmid-6 × 106,45 × 106,and 65 × 106.The strains had 8 genomovars,and were given priority to genomovar 8 (109 strains),secondly,genomovar 32 (33 strains),genomovar 5 (20 strains),genomovar 1b(i4 strains),genomovar 44 (2 strains),genomovar 7 (1 strain),genomovar 37 (1 strain),and genomovar 49 (1 strain).Among the 181 Yersinia pestis strains,strains with genes related to streptomycin resistance,sulfanilamide resistance,beta lactam resistance and disinfectant resistance were not found;and 75 of 79 strains were velogenic strains by toxicity test (MLD ≤ 10 000),accounted for 94.94％ (75/79).Conclusion The strains separated in Haixi Prefecture,Qinghai Province have the characteristics of Qinghai-Tibet Plateau plague's pathogen and have strong toxicity;all strains don't have the characteristics of drug and disinfectant resistance genes.
