Inhibitory effect and mechanism of Nultin-3 combined with cisplatin on oral squamous cell carcinoma
10.11904/j.issn.1002-3070.2018.06.002
- VernacularTitle:Nultin-3联合顺铂对口腔鳞状细胞癌细胞的抑制作用及机制研究
- Author:
Jun SHI
1
;
Xiaobing NI
;
Min MAO
;
Xingchun PENG
Author Information
1. 湖北医药学院附属太和医院口腔科 十堰 442000
- Keywords:
Nultin-3;
Cisplatin;
Tca8113 cells;
MDM2;
P53
- From:
Practical Oncology Journal
2018;32(6):488-492
- CountryChina
- Language:Chinese
-
Abstract:
Objective The objective of this study was to investigate the inhibitory effect of MDM2 inhibitor Nultin-3 com-bined with cisplatin on human oral squamous cell carcinoma(OSCC)Tca8113 cells and its mechanism. Methods Human OSCC Tca8113 cells were treated with Nultin-3,cisplatin,Nultin-3 combined with cisplatin,or vehicle control groups. The proliferation of Tca8113 cells was determined by thiazolyl blue(MTT)assay. The expression of MDM2,P53,Caspase-9 and Caspase-3 protein was determined by Western blot. Results The proliferative rate of OSCC ca8133 cells treated with Nultin-3 combined with cisplatin was significantly lower than that of other groups(P<0. 05). The relative expression of Caspase-9,Caspase-3 and P53 protein in the Nultin-3 combined with cisplatin was significantly higher than those of the Nultin-3 and cisplatin alone groups(P<0. 05). In addi-tion,the relative expression of MDM2 protein in the Nultin-3 combined with cisplatin group was significantly lower than that of the cisplatin and Nultin-3 alone groups(P<0. 05). Conclusion Nultin-3 combined with cisplatin has synergistic effect on oral squa-mous cell carcinoma Tca8113 cells. Nultin-3 regulates the MDM2-p53 signaling pathway and up-regulates the expression of pro-apoptotic proteins Caspase-9 and Casapase-3 to enhance the inhibition of cisplatin on oral squamous cell carcinoma,providing a solid theoretical basis for clinical research and treatment.
