Study on the mediating role of tumor suppressor gene PRDM5 in cervical squamous cell carcinoma induced by HPV16 virus

VernacularTitle:抑癌基因PRDM5在HPV16病毒致宫颈鳞癌发生过程中的作用研究
Author: Haiyan CHENG ¹ ; Jing MA ; Ziwei FENG ; Kejin HUANG ; Yuxia WANG ; Yunyan ZHANG
Author Information

1. 哈尔滨医科大学附属肿瘤医院哈尔滨 150081
Keywords: Cervical cancer; PRDM5; HPV16; shRNA
From: Practical Oncology Journal 2018;32(6):483-487
CountryChina
Language:Chinese
Abstract: Objective The aim of this study was to explore whether the down-regulation of tumor suppressor gene PRDM5 is one of the mechanisms of HPV16 virus infection leading to cervical cancer. Methods The expressions of PRDM5 protein and HPV16 E6/HPV18 E6 protein in cervical cancer tissues and normal cervical tissues were detected by immunohistochemistry. After transfected with HPV16 E6 shRNA plasmid,the expression of PRDM5 gene was detected in SiHa cells by RT-PCR and Western blot. Results The positive expression rate of HPV 16/18 E6 in cervical cancer tissues was significantly higher than that in normal cervical tissues. The expression level of PRDM5 protein in cervical cancer tissues was lower than that in normal cervical tissues. After HPV16 E6 shRNA3 was transfected into SiHa cells to interfere with the expression of HPV16 E6 gene,the expression of PRDM5 at mRNA and protein levels was up-regulated in SiHa cells. Conclusion PRDM5 may mediate the development of cervical cancer caused by HPV16 virus infection.