Effect of Microalgal Extracts of Tetraselmis suecica against UVB-Induced Photoaging in Human Skin Fibroblasts.
- Author:
Wol Soon JO
1
;
Kwang Mo YANG
;
Hee Sung PARK
;
Gi Yong KIM
;
Byung Hyouk NAM
;
Min Ho JEONG
;
Yoo Jin CHOI
Author Information
1. Department of Research center, Dong Nam Institute of Radiological & Medical Sciences, Busan, Korea. cyj5325@dirams.re.kr
- Publication Type:Original Article
- Keywords:
Tetraselmis suecica;
Ultraviolet B;
Photo aging;
Reactive oxygen species;
Anti-oxidant
- MeSH:
Antioxidants;
Collagen;
Fibroblasts;
Free Radicals;
Glutathione;
Humans;
Lipid Peroxidation;
Malondialdehyde;
Oxidoreductases;
Procollagen;
Reactive Oxygen Species;
Skin;
Superoxide Dismutase;
Superoxides
- From:Toxicological Research
2012;28(4):241-248
- CountryRepublic of Korea
- Language:English
-
Abstract:
Exposure of cells to ultraviolet B (UVB) radiation can induce production of free radicals and reactive oxygen species (ROS), which damage cellular components. In addition, these agents can stimulate the expression of matrix metalloproteinase (MMP) and decrease collagen synthesis in human skin cells. In this study, we examined the anti-photoaging effects of extracts of Tetraselmis suecica (W-TS). W-TS showed the strongest scavenging activity against 2,2-difenyl-1-picrylhydrazyl (DPPH) and peroxyl radicals, followed by superoxide anions from the xanthine/xanthine oxidase system. We observed that the levels of both intracellular ROS and lipid peroxidation significantly increased in UVB-irradiated human skin fibroblast cells. Furthermore, the activities of enzymatic antioxidants (e.g., superoxide dismutase) and the levels of non-enzymatic antioxidants (e.g., glutathione) significantly decreased in cells. However, W-TS pretreatment, at the maximum tested concentration, significantly decreased intracellular ROS and malondialdehyde (MDA) levels, and increased superoxide dismutase and glutathione levels in the cells. At this same concentration, W-TS did not show cytotoxicity. Type 1 procollagen and MMP-1 released were quantified using RT-PCR techniques. The results showed that W-TS protected type 1 procollagen against UVB-induced depletion in fibroblast cells in a dose-dependent manner via inhibition of UVB-induced MMP-1. Taken together, the results of the study suggest that W-TS effectively inhibits UVB-induced photoaging in skin fibroblasts by its strong anti-oxidant ability.
