Effect of 1,25-dihydroxyvitamin D3 on interleukin 17 expression in mice with asthma
10.3760/cma.j.issn.2095-428X.2014.04.017
- VernacularTitle:1,25二羟基维生素D3对支气管哮喘小鼠白细胞介素-17表达的影响
- Author:
Tongling XIE
1
;
Rongjun LIN
;
Jianping GUO
;
Mei XU
Author Information
1. 266003,青岛大学医学院儿科学教研室
- Keywords:
1,25-dihydroxyvitamin D3;
Asthma;
Interleukin 17;
Mouse
- From:
Chinese Journal of Applied Clinical Pediatrics
2014;29(4):307-310
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of interleukin 17 (IL-17) and 1,25-dihydroxyvitamin D3 [1,25-(OH)2VD3] on the pathogenesis of asthma.Methods Thirty-two Kunming mice were randomly divided into 4 groups:the control group (group A),the asthma group (group B),the 1,25-(OH)2VD3-treated group (group C) and the Dexamethasone-treated group (group D).The allergic mouse models were established by using ovalbumin (OVA).The behavioral changes of mice were observed and the number of leukocytes in the bronchoalveolar lavage fluid (BALF) were counted.HE staining was used to measure the histological changes of lung tissue.The levels of IL-17 and IgE in the BALF were detected by enzyme-linked immunosorbent assay (ELISA).Results The asthmatic symptoms were more severe in group A than those in the other groups,while they were relieved significantly in group C and group D when treated with 1,25-(OH)2VD3 or Dexamethasone.The number of leukocyte in groups B,C and D [(1.97 ± 0.23) × 108/L,(1.02 ±0.17) × 108/L,(0.95 ±0.14) × 108/L]were higher than those in group A[(0.56 ±0.16) × 108/L] (F =85.58,P ＜ 0.01),and lower in group C and group D than those in group B (all P ＜ 0.01),but there were no significant differences between group C and group D(P ＞0.05).The levels of IgE and IL-17 in group B were significantly higher than those in group A(all P ＜ 0.01).The levels of IgE and IL-17 in group B were significantly lower than those in group C and group D,but higher than those in group A(all P ＜0.01).No significant results were observed between group C and group D (all P ＞ 0.05).Conclusions IL-17 involves in the inflammation process of asthma,which promotes the respiratory inflammation.1,25-(OH)2VD3 may suppress IL-17 expression to relieve the respiratory inflammation in acute asthmatic mice,the effect of which is similar to that of Dexamethasone on asthma.
