Chemosensitivity and angiogenic potency of pre-B acute lymphoblastic leukemia cell line Sup-B15
10.3760/cma.j.issn.2095-428X.2014.03.008
- VernacularTitle:前B急性淋巴细胞白血病细胞Sup-B15对化疗药物敏感性及侵袭力的研究
- Author:
Fen ZHOU
1
;
Runming JIN
;
Yining QIU
;
Yunyun XU
;
Yi XIA
Author Information
1. 华中科技大学同济医学院附属协和医院儿科
- Keywords:
Sup-B15;
Nalm-6;
Chemosensitivity;
Invasiveness
- From:
Chinese Journal of Applied Clinical Pediatrics
2014;29(3):186-189
- CountryChina
- Language:Chinese
-
Abstract:
Objective It has been reported that Ik6 overexpression is associated with poor outcome of childhood pre-B acute lymphoblastic leukemia and Sup-B15 cell line is Ik6 positive.There were fewer studies on the Sup-B15 in the country,so the aim of this research is to explore chemosensitivity and angiogenic potency of pre-B acute lymphoblastic leukemia cell line Sup-B15 associated with Ik6 positive expression through making a comparison between Sup-B15 and Nalm-6 cell line.Methods The Ik6 mRNA and protein expression were detected by nested reverse transcription polymearse chain reaction and Western blot.The effect of vincristine,daunorubicin,L-asparaginase and dexamethasone on the proliferation of cells was measured by using CCK-8 assay.The migration and invasion of cells were detected by transwell assay and mRNA expression of angiogenesis genes (VEGF,Flt-1,PlGF,IGF-1,Ang1 and Ang2) was measured by real-time PCR.Results No expression of Ik6 was detected in Nalm-6 cell line.Compared with Nalm-6,Sup-B15 cell line had a lower rate of growth.The 4 drugs could inhibit the proliferation of cells in a dose-and time-depen-dence manner.Sup-B15 was more sensitive to daunorubicin,L-asparaginase and dexamethasone,and the folds of respective IC50 value were 9.5,1.1 and 1.5.But Sup-B15 was more resistant to vincristine than Nalm-6.The result of transwell assay showed that less Sup-B15 cells transmigrated to the lower chamber.A significant lower expression level of angiogenesis genes was detected in Sup-B15 cells.Conclusion These findings suggest that Sup-B15 is more sensitive to chemotherapy and less angiogenic compared with Nalm-6.
