Effect of arctigenin on apoptosis of mouse erythroleukemia FBL-3 cells and its mechanism
10.3760/cma.j.issn.2095-428X.2013.24.017
- VernacularTitle:牛蒡子苷元对小鼠红白血病FBL-3细胞凋亡的影响及相关机制
- Author:
Xi QIAN
1
;
Jing CUI
;
Yu WU
;
Lin-Lin FU
;
Hong WANG
Author Information
1. 河南中医学院第一附属医院药学部
- Keywords:
Arctigenin;
Leukemia;
Apoptosis;
Anti-apoptosis gene;
Pro-apoptosis gene
- From:
Chinese Journal of Applied Clinical Pediatrics
2013;28(24):1895-1897
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of arctigenin on the apoptosis of FBL-3 cells and its mechanisms.Methods The mouse erythroleukemia FBL-3 cells were taken as subjects.The untreated FBL-3 parental cells were taken as the control group,and the FBL-3 cells treated with 20 mg/L arctigenin for 24 h were taken as the experimental group.The effects of arctigenin on the apoptosis of the mouse erythroleukemia FBL-3 cells were determined by agarose gel electrophoresis and flow cytometry assay.The changes of apoptosis-related genes were analyzed by reverse transcriptase-polymerase chain reaction(RT-PCR).Results The agarose gel electrophoresis results revealed that the " DNA ladder" was displayed in the experimental group compared to the control group.The flow cytometry data demonstrated that the apoptosis number of FBL-3 cells in the experimental group was markedly increased compared to that in the control group (t =60.681,P =0.000).The RT-PCR assay results suggested that the expressions of Bcl-2 and IAP-1 gene were down-regulated in the experimental group compared to the control group (t =14.732,29.702,all P =0.000),while the expressions of Bax and Smac gene were up-regulated(t =6.721,8.499;P =0.003,0.001),but the expression of Bcl-XL did not change(t =0.209,P =0.844).Conclusions Arctigenin can induce the apoptosis of the mouse erythrolenkemia FBL-3 cells.Apoptosis of the mouse erythrolenkemia FBL-3 cells induced by arctigenin may be correlated to the down-regulation of Bcl-2 and IAP-1 and up-regulation of Bax as well as Smac.
