Expressions of 1,25 (OH)2D3, vitamin D receptor and 24-hydroxylase in Henoch-Sch(o)nlein purpura
10.3760/j.issn.2095-428X.2013.21.013
- VernacularTitle:过敏性紫癜患儿血浆1,25(OH)2D3、维生素D受体和24-羟化酶表达的意义
- Author:
Gui-Mei GUO
1
;
Juan WANG
;
Min XIA
;
Sheng HAO
;
Guang-Hua ZHU
;
Wen-Yan HUANG
;
Wei-Xun HE
Author Information
1. 200040,上海市儿童医院上海交通大学附属儿童医院肾脏风湿科
- Keywords:
Henoch-sch(o)nlein purpura;
1,25-dihydroxyvitamin D3;
Vitamin D receptor;
24-hydroxylase
- From:
Chinese Journal of Applied Clinical Pediatrics
2013;28(21):1640-1642
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expressions of 1,25 (OH) 2 D3,vitamin D receptor (VDR) and 24-hydroxylase (CYP24A1) and investigate the effects of 1,25 (OH)2D3 and its related molecules in the pathogenesis of Henoch-Sch(o)nlein purpura (HSP).Methods 1.The levels in the plasma 1,25 (OH) 2 D3 of 35 HSP patients and 14 healthy children were detected by enzyme-linked immunosorbent assay (ELISA).2.Total RNA of peripheral blood were extracted and transcribed into cDNA.Sybr green dye based real-time quantitative PCR method was used to compare the expression levels (indicated as 2-△ct value) of VDR and CYP24A1 in patients with HSP and those in the controls.Results The level of 1,25 (OH)2D3 was (13.29 ± 10.12) μg/L in plasma of HSP patients,lower than that of the healthy control group[(29.51-± 23.06) μg/L] (P ＜ 0.01).Compared with healthy control group,the level of VDR mRNA was higher but CYP24A1 mRNA was lower in HSP patients (P ＜ 0.05).Conclusion The patients with HSP have lower ability to synthesize active form of vitamin D and respond to VDR-mediated vitamin D effects,enhancing the ability to degrade this hormone.
