Relationship between cyclic adenosine monophosphate/protein kinase A pathway and glutamine-stimulated insulin secretion
10.3969/cma.j.issn.2095-428X.2013.20.006
- VernacularTitle:环磷酸腺苷/蛋白激酶A通路与谷氨酰胺刺激胰岛素分泌机制的相关性
- Author:
Yan-Mei SANG
1
;
Wen-Li YANG
;
Zi-Di XU
;
Ya-Nan ZHANG
;
Jie YAN
;
Min LIU
Author Information
1. 儿科学国家重点学科
- Keywords:
Insulin secretion;
Glutamine;
Cyclic adenosine monophosphate/protein kinase A pathway
- From:
Chinese Journal of Applied Clinical Pediatrics
2013;28(20):1537-1539
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between cyclic adenosine monophosphate/protein kinase A (cAMP/PKA) pathway and glutamine-stimulated insulin secretion.Methods In the prerequisite of the existence of glucose(0.25 mmol/L),the insulin secretion of βHC9 cells was stimulated with different concentrations of glutamine (0.0,0.5,1.0,2.0,5.0 mmol/L),then culture liquids were extracted and centrifugalized,and the insulin levels in the cell culture liquids and the cAMP levels in βHC9 cells were determined,so as to study the effects of glutamine stimulation on the insulin level in cell culture liquids and cAMP levels in βHC9 cells were assayed.Results In the prerequisite of the glucose existence,with the increasing of the concentrations of glutamine(0.0,0.5,1.0,2.0,5.0 mmol/L),the insulin levels[0.0 ng/(mL · million),19.1 ng/(mL · million),29.1 ng/(mL · million),30.1 ng/(mL · million),33.9 ng/(mL · million)] in cell culture liquids and the cAMP levels (0.0 pmol/million,40.0 pmol/million,51.5 pmol/million,52.5 pmol/million,61.3 pmoL/million) in βHC9 cells increased accordingly.Conclusion Glutamine has amplifying effect on glucose stimulated insulin secretion,such amplifying effect needs the existence of cAMP to be prerequisite.
