Effect of overexpression of LYRM1 gene on reactive oxygen species in skeletal muscle cells
10.3969/cma.j.issn.2095-428X.2013.19.009
- VernacularTitle:LYRM1基因过表达对骨骼肌细胞活性氧的影响
- Author:
Min ZHANG
1
;
Chun-Zhao KOU
;
Chun-Mei ZHANG
;
Guan-Zhong ZHU
;
Chen-Bo JI
;
Xi-Rong GUO
;
Xin-Guo CAO
Author Information
1. 210004,南京医科大学附属南京妇幼保健院儿童保健科
- Keywords:
Obesity;
LYRM1 gene;
Skeletal muscle cell;
Mitochondrial;
Reactive oxygen species
- From:
Chinese Journal of Applied Clinical Pediatrics
2013;28(19):1467-1469
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of LYRM1 overexpression on production of reactive oxygen species (ROS) in skeletal muscle cells.Methods Rat myoblasts(L6) were transfected with either an empty vector or a LYRM1 expression vector.Cells were screened and the expression of LYRM1 protein in cells was identified.L6 cells were incubated in culture solution with H2-DCFDA after they were differentiated.Then fluorescence intensity of ROS in L6 was observed by fluorescence microscope,and the content of ROS was determined by flow cytometry.Results The relative fluorescence intensity of ROS in L6 overexpressing LYRM1 was 24.8933 ± 4.4574,while that in contrast cells was 13.1512 ± 0.7347,the difference between them was significant(t =24.12,P =0.00).Conclusions Overexpression LYRM1 can increase the production of ROS in skeletal muscle cells.LYRM1 overexpression may be influence the mitochondrial function and induce the mitochondrial damage of skeletal muscle cells.
