Changes of mobility of kidney tubular epithelial cells in the course of epithelial-mesenchymal transition
10.3760/cma.j.issn.2095-428X.2013.17.007
- VernacularTitle:肾小管上皮-间充质细胞转化过程中细胞迁移率的变化
- Author:
Xiu-Ling ZHANG
1
;
Xin-Yu KUANG
;
Xiao-Ling NIU
;
Wen-Yan HUANG
Author Information
1. 青岛市妇女儿童医院
- Keywords:
Epithelial-mesenchymal transition;
Renal tubulointerstitial fibrosis;
Chronic kidney disease;
Transforming growth factor-β1;
Cell cycle
- From:
Chinese Journal of Applied Clinical Pediatrics
2013;28(17):1302-1305
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the dynamic changes of cell mobility of renal tubular epithelial cells in the course of epithelial-mesenchymal transition(EMT) and their effect on cell cycle.Methods NRK-52E cells were cultured in vitro and treated with 5 μg/L transforming growth factor(TGF)-β1 to induce EMT.The cell mobility was assessed by using Transwell chamber assay and flow cytometry (FCW) after being treated with TGF-β1 for 4 h,8 h,12 h,24 h and 48 h.The proliferative cell cycle of NRK-52E cells were evaluated by using the FCW.Results 1.EMT was successfully induced by TGF-β1.After being treated by TGF-β1 (5 μg/L),the morphological changes of NRK-52E cells were found with loose cell arrangement and elongated fusiform change in cells body.Meanwhile,after getting treated by TGF-β1,the expressions of E-cadherin protein(epithelial marker) of NRK-52E cells were significantly decreased with time-dependent (P ＜ 0.05),while the expressions of α-smooth musle actin (α-SMA) (mesenchymal cell marker)were significantly increased with time-dependent (P ＜ 0.05).2.The Transwell chamber assay showed that compared with the control group,the cell mobility in the group treated with TGF-β1 was significantly enhanced from 12 h after getting treated with TGF-β1 (P ＜ 0.01).3.The proliferative cell cycle of NRK-52E cells showed no significant difference after being treated with TGF-β1 (P ＞ 0.05).Conclusions The migration ability of the NRK-52E cells are increased incessantly in the course of EMT,which is induced by TGF-β1 without the influence of cell proliferation in vitro.
