Expression of Cysteinyl leukotriene receptor 1 in bronchial epithelial cell regulated by tumor necrosis factor-α
10.3760/cma.j.issn.2095-428X.2013.16.007
- VernacularTitle:肿瘤坏死因子-α对半胱氨酰白三烯受体1在支气管上皮细胞表达的调控
- Author:
Wei-Lin OU
1
;
Shi-Jie ZHANG
;
Xiao-Dan SONG
;
Chun-Jiang ZHU
;
Yan-Rong JI
;
Hui-Min MA
;
Bi-Wen MO
Author Information
1. 541001,桂林医学院附属医院儿科
- Keywords:
Bronchial epithelial cell;
Cysteinyl leukotriene receptor 1;
Tumor necrosis factor-α;
Modulation
- From:
Chinese Journal of Applied Clinical Pediatrics
2013;28(16):1221-1223
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore whether TNF-α involves in the modulation of Cysteinyl leukotriene receptor 1 (CysLT1) expression in bronchial epithelial cells.Methods The bronchial epithelial cell lines 16HBE cells were stimulated with different concentration (0.00,0.05,0.50,5.00,20.00 μg/L) of TNF-α for 48 hours,and CysLT1 mRNA in 16HBE cells was measured by reverse transcription(RT)-PCR.CysLT1 expression was detected by immunohistochemistry.Results 16HBE cells did not express CysLT1,after the cells were treated with TNF-α,obvious expression of CysLT1 were detected by immunohistochemistry.The weak CysLT1 mRNA expression was observed by RT-PCR in 16HBE cells,and after the cells were treated with TNF-α for 48 hours,CysLT1 mRNA expression were upregulated.When the concentrations of TNF-α were 0.00,0.05,0.50,5.00,and 20.00 μg/L respectively,the relative intensities of CysLT1 mRNA/β-actin were 0.048,0.105,0.177,0.182,0.495,respectively.Conclusions TNF-α can upregulate CysLT1 mRNA expression in 16HBE ceils in a dose-dependent manner.When infected by virus,respiratory tract produces abundant TNF-α.The TNF-α can upregulate the expression of CysLT1 in epithelial cells,enhance inflammation reaction in respiratory tract.This may explain partially the mechanism of exacerbation of asthma induced by respiratory tract infection.
