Prognostic significance of Notch1 protein expression in children with pediatric B-cell acute lymphoblastic leukemia
10.3760/cma.j.issn.2095-428X.2013.15.007
- VernacularTitle:Notch1蛋白表达在儿童急性B淋巴细胞白血病预后预测中的意义
- Author:
Xiao-Yun ZHAN
1
;
Yan SUN
;
Shuang-You LIU
;
Guan-Ling WANG
;
Qun HU
;
Ai-Guo LIU
;
Liu-Qing ZHANG
Author Information
1. 华中科技大学同济医学院附属同济医院儿科
- Keywords:
Acute lymphoblastic leukemia;
B lymphocyte;
Notch1 protein;
Prognosis
- From:
Chinese Journal of Applied Clinical Pediatrics
2013;28(15):1138-1141
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate Notch1 protein expression of leukemic cells in children with pediatric Bcell acute lymphoblastic leukemia(B-ALL) and its effect on prognosis.Methods The expression of Notch1 protein of leukemic cells in bone marrow smears was detected by immunohistochemistry(SABC) in primarily diagnosed childhood ALL,including 63 cases of B-ALL and 14 cases of T-ALL,a group of 34 children with no malignancy served as controls.Reverse transcription-ploymerase chain reaction was used to assay Notch1 mRNA expression in ALL patients.Results The incidence of Notch1 expression was 31.7％ in B-ALL patients,71.4％ in T-ALL patients,and 8.8％ in control group,respectively.Notch1 protein was aberrantly expressed in both B-ALL and T-ALL compared with the controls(P ＜ 0.05,P ＜ 0.001).Multivariate analysis revealed that the expression of Notch1 protein in B-ALL was not associated with patients' age,gender,WBC count at diagnosis(all P ＞ 0.05),it had no influence on the early treatment response.Nevertheless,the Kaplan-Meier curve of event-free survival showed that,in the patients without Notch1 protein expression,the long-term event-free survival rate was as high as 92.7 ％,in contrast,in the children with Notch 1 expression,the event-free survival was only 54.5 ％ (P =0.0054).Conclusions The expression of Notch 1 protein in pediatric B-ALL may predict a poor prognosis,and interfering with Notch1 signaling could be employed as a potential therapeutic target for those patients with Notch1 expression.
