Effects of different mechanical ventilation methods on morphology of type Ⅱ alveolar epithelium cell in newborn piglets with acute lung injury
10.3760/cma.j.issn.2095-428X.2013.14.009
- VernacularTitle:不同机械通气方式对急性肺损伤新生猪肺泡Ⅱ型上皮细胞的影响
- Author:
Wan-Hai FU
1
;
You-Wei ZHAO
;
Xiao-Fei QIN
;
Chu-Ming YOU
;
Jian LIANG
;
Yun-En LIN
;
Wei-Biao HUANG
Author Information
1. 广东省第二人民医院儿科
- Keywords:
Mechanical ventilation;
Type Ⅱ alveolar epithelium cell;
Morphology;
Newborn piglet
- From:
Chinese Journal of Applied Clinical Pediatrics
2013;28(14):1069-1072
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of 3 different ventilation methods,including conventional mechanical ventilation(CMV),high-frequency oscillatory ventilation(HFOV) and partial liquid ventilation(PLV),on the morphology of type Ⅱ alveolar epithelium cell (ACE Ⅱ) of newborn piglet with acute lung injury (ALI).Methods Twenty-four less than 3-day-old newborn piglets were enrolled.After ALI was established with saline lavage (38 ℃,0.035 L/kg),newborn piglets were randomly assigned to 4 study groups:control group(n =6,no ventilation),CMV group(n =6),HFOV group(n =6),and PLV group(n =6,38 ℃,0.018 L/kg).Piglets in the 4 groups were sacrificed after being ventilated for 24 hours,and the number,area,density of fluorescence of ACE Ⅱ were detected.Results Through 24 hours mechanical ventilation,the numbers of ACE Ⅱ in CMV group,HFOV group and PLV group remained were 30 ± 5,52 ± 5,81 ± 7,respectively,while areas of fluorescence were 340.40 ± 47.50,329.69 ± 124.50,295.55 ± 109.30,respectively,and there were significant differences among the 3 groups,and the population mean of density of fluorescence had significant difference among the 3 groups.The number of ACE Ⅱ remains was lowest in CMV group compared with HFOV group(P =0.026) and PLV group (P =0.000),and the density of fluorescence of ACE Ⅱ was lowest in CMV group compared with HFOV group (P =0.001) and PLV group (P =0.002).Conclusions Different mechanical ventilations have various effects for ACE Ⅱ,and CMV is the most harmful mechanical ventilation on ACE Ⅱ,while PLV is the least harmful.
