Expression pattern changes of microRNA in the lung tissue of mice with acute lung injury induced by lipopolysaccharide
10.3760/cma.j.issn.2095-428X.2013.12.016
- VernacularTitle:脂多糖诱发的小鼠急性肺损伤肺组织中microRNA表达谱的变化
- Author:
Bin WNAG
1
;
Di QING
;
Dong-Liang CHENG
;
Shao-Hua TAO
;
Wei SHEN
;
Yan-Chen CHEN
;
Jiang DU
Author Information
1. 南方医科大学珠江医院儿科
- Keywords:
microRNA;
Acute lung injury;
Acute respiratory distress syndrome
- From:
Chinese Journal of Applied Clinical Pediatrics
2013;28(12):931-935
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the change of expression pattern of microRNA in the lung with acute lung injury (ALI)/acute respiratory distress syndrome(ARDS) induced by lipopolysaccharide (LPS),and to provide an evidence that microRNAs are involved in the pathogenesis of ALI/ARDS.Methods Twenty-four C57BL mice were randomly divided into control group and LPS treated group,12 mice in each group.The rats in LPS treated group were treated with intratracheal injection of LPS at a dose of 10 mg/kg body weight into the lung.The rats in control group were treated with the same dose of saline instead.All mice were sacrificed 24 hours after operation,the left lung was excised to measure the wet-to-dry weight (W/D) ratio,and the right upper lobe was stained with hematoxylin and eosin (HE).A microRNA microarray chip was used to profile miRNA expressions in the lung of rats in both LPS treated group and control group.Online software packages were used to predict the gene targeted by microRNAs.Results Compared with the control group,the LPS treated mice had obvious respiratory symptoms,the W/D ratio was significantly increased (P ＜ 0.01),and the pathology was characterized with ALI/ARDS.The microarray chip results demonstrated that the expressions of 48 microRNAs were significantly changed in the ALI/ARDS mice.Among these miRNA,27 cases were up-regulated,21 cases were down-regulated.The target genes of these microRNAs might be involved in regulating the signal pathway of inflammation.Conclusions Some miRNAs express differently in the model of ALI/ARDS,and they may play an important role in pathophysiological process of ALI/ARDS.
