Effect of progesterone on expression of brain tissue damage factors and anti damage factors in neonatal rats with hypoxic-ischemic brain damage
10.3760/cma.j.issn.2095-428X.2013.12.013
- VernacularTitle:孕酮对缺氧缺血性脑损伤新生大鼠脑组织损伤因子和抗损伤因子表达的影响
- Author:
Xiao-Yin WANG
1
;
Xiao-Hong HAN
;
Dao-Wen SI
;
Ru-Ling SHI
;
Xue-Peng GUO
Author Information
1. 453003,新乡医学院生物化学与分子生物学教研室
- Keywords:
Progesterone;
Hypoxic-ischemic brain damage;
Damage factor;
Anti damage factor;
Neonatal rat
- From:
Chinese Journal of Applied Clinical Pediatrics
2013;28(12):920-922
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of progesterone on tumor necrosis factor (TNF-α),interleukin-1β (IL-1β),nerve growth factor(NGF) and brain derived neurotrophic factor(BDNF) expression in the cortex and hippocampus tissue in newborn rats with hypoxic-ischemic brain damage (HIBD),and to discuss the protective molecular mechanism of progesterone on HIBD in neonatal rats.Methods Forty-eight 7-day-old neonatal rats were randomly divided into 3 groups:sham-operated group,hypoxic-ischemic group and pretreatment group.Rats in hypoxic-ischemic group and pretreatment group were subjected to left common carotid artery ligation,then they were exposed to 80 mL/L oxygen and 920 mL/L nitrogen gas in the closed container at 37 ℃ for up to 2.5 h to establish HIBD models.Progesterone was injected intraperitoneally into the rats in the pretreatment group 30 min before hypoxia,and solution was injected into the first 2 groups.All the rats were killed at the 24 h after operation.The levels of TNF-α,IL-1β,NGF,BDNF were measured by enzyme linked immunosorbent assay and the expressions of TNF-α,IL-13,NGF,BDNF mRNA were analyzed by reverse transcription-polymerase chain reaction.Results The contents of TNF-α,IL-1β,NGF,BDNF and their mRNA expressions in hypoxic-ischemic group were significantly higher than those in the sham-operated group.In pretreatment groups,the levels of TNF-α,IL-1β and their mRNA expressions were significantly lower than those in hypoxic-ischemic group.The levels of NGF,BDNF and their mRNA expressions in pretreatment group were significantly higher than those in hypoxic-ischemic group (all P ＜ 0.05).Conclusions Progesterone exerts neuroprotective effect on hypoxic-ischemic encephalopathy-induced brain damage,and the action mechanism is related to down-regulate the expression of damage factor and up-regulate the expression of anti damage factor.
