Expression of glucocorticoid receptor in steroid-induced juvenile rabbit model of avascular necrosis of the femoral head and its significance
10.3760/cma.j.issn.2095-428X.2013.11.011
- VernacularTitle:糖皮质激素受体在激素性幼兔股骨头缺血性坏死模型中的表达及意义
- Author:
Yi XIE
1
;
Song YU
;
Yu LUO
;
Yan-Peng XU
;
Gang PENG
;
Jian WANG
;
Shi-Peng TANG
Author Information
1. 563003,遵义医学院附属医院小儿矫形外科
- Keywords:
Avascular necrosis of the femoral head;
Animal model;
Glucocorticoid;
Glucocorticoid receptor;
Juvenile rabbit
- From:
Chinese Journal of Applied Clinical Pediatrics
2013;28(11):835-838
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of glucocorticoid receptor(GR) in juvenile rabbit modal of avascular necrosis of the femoral head,and explore the possible etiology of avascular necrosis of the femoral head in children.Methods Sixty New Zealand albino rabbits(50％ female and 50％ male),2 months old,body weight 1.4-1.7 kg,were randomly divided into 2 groups:steroid injection group (SIG) and control group(CG).The SIG(n =48):Prednisolone acetate of 7.5 mg/kg was injected into each rabbit twice a week for 8 weeks.The CG(n =12):9 g/L saline of 0.3 mL/kg was injected into each rabbit twice a week for 8 weeks.The immunohistochemistry and image analysis were used to detect the expression of GR.The SIG was divided into disease group(DG) and non-disease group(NDG).The expressions of GR were compared among DG,NDG and CG.Results In the 8th week after the first injection in SIG,31 out of 48 rabbits survived,and 7 out of 31 rabbits(22.85％) developed femoral head avascular necrosis.GR expression in DG was 100.00％ (7/7 cases),while in NDG and CG they were 20.83％ (5/24 cases) and 16.67％ (1/6 cases).Significant difference was found between DG and NDG/CG(all P ＜ 0.01),but not between NDG and CG.GR expression density in femoral cartilage in DG was higher than in NDG and CG(all P ＜ 0.01).GR expression IOD in femoral cartilage was 7942.21 ± 1886.94,but in DG and NDG they were extremely low or undetectable,which were 627.56 ± 281.85 and 571.48 ± 260.22,separately,and there was significant difference (all P ＜ 0.01).Conclusions Simple gluteal injection of glucocorticoid can lead to juvenile rabbits avascular necrosis of femoral head.GC and GR might play an important role in the onset and development of avascular necrosis of femoral head in juvenile rabbits.
