Evaluation of the Usefulness of Selective Chromogenic Agar Medium (ChromID VRE) and Multiplex PCR Method for the Detection of Vancomycin-resistant Enterococci.
10.3343/kjlm.2010.30.6.631
- Author:
Do Hoon KIM
1
;
Jae Hee LEE
;
Jung Sook HA
;
Nam Hee RYOO
;
Dong Seok JEON
;
Jae Ryong KIM
Author Information
1. Department of Laboratory Medicine, Keimyung University School of Medicine, Daegu, Korea. nhryoo@dsmc.or.kr
- Publication Type:Original Article ; Comparative Study ; English Abstract ; Evaluation Studies
- Keywords:
VRE;
Chromogenic agar;
PCR
- MeSH:
Agar/chemistry;
Chromogenic Compounds/*chemistry;
Enterococcus/drug effects/genetics/*isolation & purification;
Enterococcus faecium/genetics/isolation & purification;
Feces/microbiology;
Genotype;
Humans;
Phenotype;
Polymerase Chain Reaction/*methods;
Reagent Kits, Diagnostic;
*Vancomycin Resistance
- From:The Korean Journal of Laboratory Medicine
2010;30(6):631-636
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Accurate and early detection of vancomycin-resistant enterococci (VRE) is critical for controlling nosocomial infection. In this study, we evaluated the usefulness of a selective chromogenic agar medium and of multiplex PCR for detection of VRE, and both these techniques were compared with the conventional culture method for VRE detection. METHODS: We performed the following 3 methods for detecting VRE infection in stool specimens: the routine culture method, culturing in selective chromogenic agar medium (chromID VRE, bioMerieux, France), and multiplex PCR using the Seeplex(R) VRE ACE Detection kit (Seegene Inc., Korea) with additional PCR for vanC genes. RESULTS: We isolated 109 VRE strains from 100 stool specimens by the routine culture method. In chromID VRE, all the isolates showed purple colonies, including Enterococcus gallinarum and E. raffinosus, which were later identified using the Vitek card. All VRE isolates were identified by the multiplex PCR method; 100 were vanA-positive E. faecium, 8 were vanA- and vanC-1-positive E. gallinarum, and 1 was vanA-positive E. raffinosus. CONCLUSIONS: For VRE surveillance, culturing the isolates in chromID VRE after broth enrichment appears to be an accurate, rapid, and easy method for routine screening test. Multiplex PCR is relatively expensive and needs skilled techniques for detecting VRE, but it can be an auxiliary tool for rapid detection of genotype during a VRE outbreak.
