Expression patterns of Th1 and Th2 cytokine genes in childhood acute idiopathic thrombocytopenic purpura
10.3760/cma.j.issn.2095-428X.2013.03.016
- VernacularTitle:急性特发性血小板减少性紫癜患儿Th1/Th2类细胞因子的基因表达水平
- Author:
Jin-Shen WANG
1
;
Yue-Qin HAN
;
Li-Xia WEI
;
Dao-Gang QIN
;
Qiao-Zhi YANG
Author Information
1. 252000,聊城市人民医院儿科
- Keywords:
Idiopathic thrombocytopenic purpura;
Type Ⅰ and type Ⅱ helper T cells;
Cytokine;
Gene
- From:
Chinese Journal of Applied Clinical Pediatrics
2013;28(3):214-216
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression patterns of Th1 and Th2 cytokine genes in childhood acute idiopathic thrombocytopenic purpura(ITP) and its clinical value.Methods Peripheral blood samples from 30 patients with acute ITP before and after treatment (ITP group) and those from 20 normal healthy subjects (healthy control group) were collected (healthy control group),and reverse transcriptases polymerase chain reaction was performed to determine the mRNA expressions of Th1 and Th2 cytokine genes before and after treatment,which were compared with those of the health controls.Results The positive rate of expression levels of Th1 cytokine genes in samples from ITP patients (3.3％,3.3％) were significantly lower than those from healthy control group (all P ＜ 0.05) and increased to normal level after treatment(16.7％,23.3％).In contrast,Th2 cytokine genes (IL-4,IL-6,IL-10)in the samples from the ITP patients(33.3％,43.3％,40.0％) were significantly higher than those from the healthy control group (all P ＜0.05) and decreased after treatment(10.0％,23.3％,20.0％).Conclusions Such data indicate that ITP is a Th2 cell predominant autoimmune disease,and the abnormal immunity due to Th1/Th2 shift is significant in the pathogenesis of ITP.
