Analysis of a G1793A polymorphism of methylenetetrahydrofolate reductase gene in children with acute leukemia
10.3760/cma.j.issn.2095-428X.2013.03.010
- VernacularTitle:急性白血病患儿MTHFR基因G1793A多态性的相关研究
- Author:
Miao-Miao ZHENG
1
;
Li-Jie YUE
;
Chun-Lan YANG
;
Cai XIE
;
Hui DING
Author Information
1. 遵义医学院深圳市儿童医院儿科研究所
- Keywords:
Methylenetetrahydrofolate reductase;
Single nucleotide polymorphism;
Acute leukemia;
Child
- From:
Chinese Journal of Applied Clinical Pediatrics
2013;28(3):191-194
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution of methylenetetrahydrofolate reductase (MTHFR) gene G1793A genotype and allele frequency in children with acute leukemia (AL),and to analyze the association between MTHFR gene polymorphism and the risk of AL.Methods Bone marrow samples from 109 childhood patients with AL and peripheral blood samples from 120 control children were obtained to prepare complementary DNAs (cDNAs).The cDNAs were analyzed for MTHFR G1793A polymorphism by reverse transcriptase-polymerase chain reaction-denaturing gradient gel electrophoresis and sequencing.Results The frequencies of 1793 GG,GA and AA genotyp(s) in MTHFR of the AL patients,acute lymphoblastic leukaemia (ALL) patients,acute myeloid leukaemia (AML) patients and the control children were 83.5％,15.6％,0.9％ ;82.8％,16.1％,1.1％ ;86.4％,13.6％,0％ and 89.2％,7.5％,3.3％,respectively.While the A allele frequency of MTHFR G1793A in those group were 8.7％,9.2％,6.8％ and 7.1％,respectively.However,no significant difference was observed in MTHFR G1793A genotypes or A allele frequency between the patients and controls (all P ＞0.05).The MTHFR 1793 GA + AA genotype was linked with an increased risk of AL,ALL and AML (AL:OR =1.71,95％ CI:0.77-3.80,P =0.19;ALL:OR =2.00,95％ CI:0.85-4.49,P =0.12;AML:OR =1.36,95％CI:0.33-5.62,P =0.67),but no significant difference in our population(all P ＞ 0.05).The A allele frequency of MTHFR G1793A in the study was 7.9％,significantly different from those reported in Ashkenazi Jewish,African-American,Brazilian,Austrian,Irau and Harbin populations (all P ＜0.05).Conclusion MTHFR G1793A may be not a genetic susceptibility factor for pediatric AL,but may exhibit an ethnic difference.
