The expression and clinical significance of hypoxia-inducible factor-1α in patients with traumatic brain injury
10.3760/cma.j.issn.1671-0282.2018.12.005
- VernacularTitle:创伤性脑损伤后缺氧诱导因子-1α 的表达
- Author:
Bing LI
1
;
Yuanli YANG
;
Meixia WANG
;
Feng CAO
;
Ronglin CHEN
;
Hongxuan ZHANG
Author Information
1. 深圳市龙岗中心医院重症医学科
- Keywords:
Hypoxia-inducible factor-1α;
Traumatic brain injury;
Reverse Transcription-Polymerase Chain Reaction;
Western blot
- From:Chinese Journal of Emergency Medicine
2018;27(12):1337-1340
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of hypoxia-inducible factor-1α (HIF-1α) in patients with traumatic brain injury (TBI) and their clinical significance. Methods Peripheral blood and brain tissue samples were obtained from 60 TBI patients. According to the GCS score, 60 TBI patients were divided into the moderate damage group, the severe damage group and the especially severe damage group. According to the different time points after the injury, the patients were divided into <6 hours group, 6-24 hours group, 24-72 hours group and >72 hours group. The 60 control brain tissue samples were obtained from patients with cerebral aneurysms and undergoing craniotomy at the same time; and control peripheral blood were collected from 60 healthy people. The levels of HIF-1α were measured with RT-PCR and Western blot . One-way ANOVA and t-test were used to analyze the results with SPSS 18.0. Results The expression of HIF-1α in the control group [peripheral blood: HIF-1α mRNA (0.35±0.12), HIF-1α protein (0.28±0.06) ;brain tissue: HIF-1α mRNA (0.65±0.08),HIF-1α protein (0.78±0.08)] was obviously lower than those in the TBI groups, and the differences were statistically significant (P<0.05). Along with the damage degree aggravating, the expression of HIF-1α was increased. The expression of HIF-1α in the especially severe damage group was statistically higher than those of the severe damage group and the moderate damage group (P<0.05). The expression of HIF-1α was increased along with the extension of time after the injury. The expression of HIF-1α in the 24-72 h group was significantly higher than those of the >72 h group, 6-24 h group and <6 h group (P<0.05). Conclusions The expression of HIF-1α is closely related to the severity of TBI and may play an important role in the progress of TBI.
