Effect of Yanghe decoction serum on the proliferation of breast cancer stem cells
10.3760/cma.j.issn.1673-4246.2019.01.012
- VernacularTitle:阳和汤含药血清对乳腺癌干细胞增殖的影响
- Author:
Kai YE
1
;
Fenli HE
;
Xiaojuan YANG
;
Rongguo SHANG
;
Kangle LI
;
Jiansheng QIAN
;
Jianwei DOU
Author Information
1. 甘肃中医药大学基础医学院
- Keywords:
Neoplastic stem cells;
Cell proliferation;
Receptor,EphA4;
NF-kappa B p50 subunit;
Yanghe decoction;
Rats
- From:
International Journal of Traditional Chinese Medicine
2019;41(1):49-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Yanghe decoction serum on the proliferation of breast cancer stem cells HMLER90hi and its mechanism. Methods Twenty female Sprague-Dawley rats were randomly divided into low,medium and high dose groups containing drug serum and control group, in order to prepare the Yanghe decoction serum and blank serum. After 24 hours of drug intervention,the effects of each group on the proliferation of HMLER90hi cells at 24 h,48 h,and 72 h were detected by MTT assay. The expression of EphA4 and p50 mRNA in each group were detected by real-time fluorescence quantitative PCR. Results Compared with the blank control group,the cell proliferation activity of each dose group of Yanghe decoction significantly decreased at 24 h (0.818 ± 0.061, 0.706 ± 0.073, 0.587 ± 0.052 vs. 0.928 ± 0.075), 48h (0.760 ± 0.047, 0.638 ± 0.056, 0.510 ± 0.059 vs. 0.973 ± 0.095), and 72 h (0.672 ± 0.102, 0.508 ± 0.092, 0.448 ± 0.048vs.1.023 ± 0.099) (P<0.05 orP<0.01), respectively. After 24 h of drug intervention, compared with the control group, the expression of EphA4 mRNA (0.54 ± 0.07, 0.54 ± 0.07, 0.33 ± 0.04 vs.0.68 ± 0.09) significantly decreased, and p50 mRNA (0.69 ± 0.10, 0.54 ± 0.08, 0.41 ± 0.06vs. 0.85 ± 0.13) significantly decreased in each dose group of Yanghe decoction (P<0.05 orP<0.01).ConclusionsTheYanghe decoction can inhibit the proliferation of breast cancer stem cell HMLER90hi,and its mechanism may be related to its inhibition of the conduction of the juxtacrine pathway of monocyte macrophage.
