Isolation and content determination of taxifolin, orobol and quercetin in Cudrania tricuspidata
10.3760/cma.j.issn.1673-4246.2018.12.019
- VernacularTitle:柘木中花旗松素、奥洛波尔、槲皮素分离鉴定与含量测定
- Author:
Jiamei ZHANG
1
;
Xiaoyu GUO
;
Qinghua QUAN
;
Ruifang JI
;
Qianqian SUN
;
Jingyun TIAN
;
Peng TAN
;
Yonggang LIU
Author Information
1. 100102,北京中医药大学中药学院
- Keywords:
Chromatography;
high pressure liquid;
Quercetin;
Taxifolin;
Orobol;
Cudrania tricuspidata
- From:
International Journal of Traditional Chinese Medicine
2018;40(12):1187-1190
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate and identify 3 flavonoids (taxifolin, orobol and quercetin) from Cudrania tricuspidata, and develop a method for determining 3 flavonoid constituents in Cudrania tricuspidata. Methods Three flavonoids was isolated from ethanol extract of Cudrania tricuspidata by chromatography, and its structure was identified by nuclear magnetic resonance. The analysis was conducted on an Aglient C18 column (4.6 mm ×250 mm, 5 μm) eluted with 1％ acetic acid and methanol as mobile phases in gradient mode. The flow rate was 1 ml/min and the detection wavelength was set at 310 nm. The column temperature was 25 ℃. Results Taxifolin, orobol and quercetin were isolated from ethanol extract of Cudrania tricuspidata by chromatography. The content of taxifolin, orobol and quercetin were 0.850 mg/g, 0.518 mg/g, 0.103 mg/g. Conclusion The method can be used for the quality control of Cudrania tricuspidata as a reference.
