Simultaneous detemination of 8 terpenoids compounds in tripterygium wilfordii suspension cells by UPLC-MS/MS
10.3760/cma.j.issn.1673-4246.2018.12.017
- VernacularTitle:UPLC-MS/MS同时测定雷公藤悬浮细胞中8种萜类成分
- Author:
Xiaoyi WU
1
;
Keyi ZHANG
;
Rui ZHANG
;
Yi JIA
;
Huifang YU
Author Information
1. 首都医科大学中医药学院
- Keywords:
Tripterygium wilfordii;
Terpenes;
Suspension cells;
Chemical quantitative analysis;
UPLC-QQQ-MS/MS
- From:
International Journal of Traditional Chinese Medicine
2018;40(12):1176-1180
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a UPLC-QQQ-MS/MS (ultra-high performance liquid chromatography triple quadrupole tandem mass Spectrometry) method for simultaneous detemination of 8 terpenoids compounds in Tripterygium wilfordii suspension cells. Methods The separation and analysis were performed on Water HSS T3 C18, with 0. 1％ acetic acid solution-acetonitrile as the mobile phase for gradient elution. The column temperature was set at 30 ℃ with flow rate was 0.3 ml/min. The electrospray ionization (ESI) source was applied and operated in alternating positive and negative mode. Multi-reaction monitor (MRM) mode was used to quantify the 8 compounds. Results The limits of detection and limits of quantitation in 8 compounds (triptolide, triptonide, dehydroabietlc acid, triptophenolide, demethylzeylasteral, tripteridine, pristimerin, celastrol) were 0.0096-0.2480 ng and 0.0192-0.4960 ng, respectively. The correlation coefficents (r) of calibration curves were 0.9966-0.9993. The average recovery rates were 95.04％-105.20％, and the relative standard deviation was 2.14％-6.31％. Conclusions The established quantitative method is simple, rapid, sensitive and accuracy. It can be used for simultaneous analysis of 8 terpenoids compounds in Tripterygium wilfordii suspension cells by using UPLC-QQQ-MS/MS, which affords methodology evidence for research and quality control of Triptervgium wilfordii Hook. f. and its plant tissue cultures.
