Usefulness of a Disk Method for Detection of Hippurate Hydrolysis by Campylobacter jejuni.
- Author:
Young UH
1
;
Soon Duk PARK
;
Hyun Mi CHO
;
Hyeun Gyeo LEE
;
An Suk JEOUNG
;
Mi Kyung LEE
;
Kap Jun YOON
Author Information
1. Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea. u931018@wonju.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Campylobacter jejuni;
Hippuricase;
PCR;
Hippurate hydrolysis;
Disk
- MeSH:
Campylobacter jejuni*;
Campylobacter*;
Diagnostic Tests, Routine;
Hydrolysis*;
Ninhydrin;
Polymerase Chain Reaction;
Sensitivity and Specificity
- From:Korean Journal of Clinical Microbiology
2002;5(1):42-46
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The test for hippurate hydrolysis is critical for differentiation of C. jejuni and other thermophilic Campylobacter species. So, we evaluated the disk method for detection of hippurate hydrolysis by C. jejuni. METHODS: Twenty-eight Campylobacter species isolated from stool culture were simultaneously tested with disk method for detection of hippurate hydrolysis and polymerase chain reaction (PCR) for hippuricase specific gene. Disk method was tested with difference in incubation time (2 hours vs. 4 hours), hippurate concentration (1%, 2%, and 4%), amount of ninhydrin (50 microliter vs. 100 microliter), and inoculation method (colony vs. suspension of organism adjusted by turbidity), finally, 24 types of disk methods were performed. RESULTS: By using hippuricase PCR method as the reference for the detection of hippurate hydrolysis, the disk method showed a sensitivity of 91.7% and a specificity of 100% when two kinds of disk methods were simultaneously performed. CONCLUSIONS: The disk method for detection of hippurate hydrolysis is simple to use and require fewer cells than the tube method do, and should be useful as a routine diagnostic test in clinical laboratory for rapid identification of C. jejuni.
