Effect of miR-758-3p on invasion and proliferation of gastric cancer cell line MGC803 by targeting MDM2
10.3760/cma.j.issn.1673-4203.2018.12.006
- VernacularTitle:miR-758-3p通过靶向作用鼠双微体基因2对胃癌细胞MGC803侵袭和增殖的影响
- Author:
Mingming DONG
1
;
Xinmin LI
;
Shujiang PENG
;
Ruizhi LIU
Author Information
1. 435000,鄂东医疗集团黄石市中心医院(湖北理工学院附属医院)胃肠外科
- Keywords:
Stomach neoplasms;
MicroRNAs;
Neoplasm invasiveness;
Cell proliferation;
Murine double microgene 2
- From:
International Journal of Surgery
2018;45(12):815-820
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the regulation of microRNA (miRNA,miR)-758-3p on the expression of murine double microsomal gene 2 (MDM2) and its effect on invasion and proliferation of gastric cancer cell line MGC803.Methods The bioinformatics software was used to predict MDM2 as target gene of miR-758-3p.The wild type MDM2 gene 3'untranslated region luciferase reporter gene vector and miR-758-3p target sequence mutated vector and the corresponding miRNA were transfected into gastric cancer cells MGC803 by lipofectamine.Dual luciferase reporter system detects luciferase activity.The miR-758-3p mimics were transfected into gastric cancer cell MGC803 by lipofectamine.Real-time PCR was used to detect the transfection efficiency.Real-time PCR and Western blot were used to detect miR-MDM2 expression level in cells after transfection.Transwell assay and CCK-8 assay were used to detect cell invasion and proliferation.SPSS 20.0 was used to conduct the statistical analysis.Results Dual luciferase reporter assay confirmed that miR-758-3p could target MDM2 gene(P ＜ 0.05).The expression level of miR-758-3p in MGC803 cells transfected with miR-758-3p mimics was significantly higher than that in miR-NC cells [(6.68 ±0.53) vs (0.84 t0.12),P ＜0.01].Compared with miR-NC group,MDM2 expression was down-regulated in MGC803 cells transfected with miR-758-3p mimics (P ＜ 0.05).The number of invasive cells in miR-NC group and miR-758-3p group were (136.00 ± 16.62) and (79.49 ± 6.42).After knockdown MDM2,the invasiveness of cells was significantly decreased (P ＜ 0.05).The results of CCK-8 showed that the proliferation of MGC803 cells transfected with miR-758-3p group was significantly lower than that of miR-NC group (P ＜ 0.01).Conclusion miR-758-3p can reduce the invasion and proliferation of MGC803 cells by targeting MDM2.
