Effects of bone barrow mesenchymal stem cells on inflammatory factors IL-1 beta and IL-10 in young rats with acute lung injury
10.3760/cma.j.issn.1673-4408.2018.12.018
- VernacularTitle:骨髓间充质干细胞对急性肺损伤幼鼠炎症因子IL-1β、IL-10的影响
- Author:
Kai LIU
1
,
2
;
Jiujun LI
;
Peng SUN
;
Qianying LI
Author Information
1. 110004 沈阳,中国医科大学附属盛京医院PICU
2. 深圳大学总医院儿科
- Keywords:
Bone marrow mesenchymal stem cell;
Acute lung injury;
Young mice;
Interleukins-1 beta;
Interleukin-10
- From:
International Journal of Pediatrics
2018;45(12):973-977,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of bone mesenchymal stem cells(BMSC) on the expression level of inflammatory factors IL-1beta and IL-10 from young rats with acute lung injury(ALI).Methods Seventy-two SPF male SD young rats were randomly divided into three groups:control group,disease model group(model group),disease model + BMSC(treatment group).Each group was then randomly divided into four subgroups according to the treatment phages.The juvenile rat ALI models were established.BMSC was injected into the treatment group,and DMEM was injected into the model and the control groups.Then,the pathological changes of lung tissues were observed.The W/D ratio,the neutrophil count and protein content in bronchoalveolar lavage fluid(BALF) were measured.The changes of IL-1beta and IL-10 contents were detected.Results Compared with the control group and the treatment group,the number of neutrophils,total protein content and W/D ratio of BALF lung tissue in the model group increased significantly in each period(P ＜ 0.05).The degree of lung injury in the treatment group was significantly weaker than that in the model group.The levels of IL-1beta and IL-10 in serum decreased in different treatment periods(48 hours vs 6 hours,P ＜0.05).In model group,IL-1beta was abundantly expressed in the cytoplasm and wall of alveolar epithelial cells,while in treatment group,the expression intensity of IL-1 beta protein was significantly decreased.The levels of IL-1 beta and IL-10 protein in lung tissues of model group were significantly higher than those of control group(P ＜0.05).Conclusion BMSC has an important effect on the expression of inflammatory factors IL-1beta and IL-10 in ALI young rates.BMSC can alleviate the inflammatory reaction of long injury and participate in the repair of lung iniury.
