Focal adhesion kinase mediated signaling on atrial fibrosis in patients with valvular atrial fibrillation
- VernacularTitle:黏着斑激酶介导信号通路在瓣膜性心房颤动心房纤维化中的研究
- Author:
ZHANG Peide
1
;
LI Fei
1
;
QIAO En
1
;
WANG Wei
1
Author Information
1. Fuwai Hospital, China Academy of Medical Science, National Center for Cardiovascular Disease, Beijing, 100037, P.R.China
- Publication Type:Journal Article
- Keywords:
Valvular atrial fibrillation;
atrial fibrosis;
focal adhesion kinase;
signaling pathway
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2019;26(4):379-384
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes of focal adhesion kinase (FAK) in the fibrotic atrium of patients with valvular atrial fibrillation and explore its downstream signaling pathways. Methods A total of 45 patients with mitral valve disease were included in this study and were divided into a valvular atrial fibrillation group (VAF, ≥6 months, 25 patients) and a sinus rhythm group (SR, 20 patients) based on having atrial fibrillation or not. The atrial appendage tissue was obtained during the operation , histopathological examination and Western blotting were performed. The degree of atrial fibrosis and changes in FAK and its downstream pathways in fibrotic myocardium were observed. Results This study revealed a higher degree of atrial fibrosis in valvular atrial fibrillation and disordered cell arrangement. Expression of fibroblast differentiation marker alpha smooth muscle actin (α-SMA) was significantly increased in atrial fibrillation, and the expression of FAK and downstream AKT/S6K pathway proteins was up-regulated, while the other signal was observed, there was no significant change in ERK1/2 signaling pathway. Conclusion Atrial fibrosis in valvular atrial fibrillation is an important feature of atrial structural remodeling. We found overproduction of collagen fibers disrupted the continuity of atrial myocytes, leading to abnormal conduction and providing a matrix environment for the development of atrial fibrillation. The expression of focal adhesion kinase and downstream AKT/S6K signaling pathway in fibrotic myocardium may be involved in the process of atrial fibrosis, providing a basis for the study of its mechanism.
